Transcription unit of the chicken histone H5 gene and mapping of H5 pre-mRNA sequences.
نویسندگان
چکیده
منابع مشابه
Mapping of histone H5 sites on nucleosomes using immunoelectron microscopy.
The location of histone H5 on nucleosomes has been determined by binding anti-H5 antibodies to dinucleosomes, and recording the position of the bound IgG molecules using electron microscopy. Two types of antibody were employed, a total IgG fraction prepared from rabbits immunized with H5/RNA, which reacted to all three domains (NH2-terminal, central globular, and COOH-terminal) of H5, and an im...
متن کاملBasal expression of the histone H5 gene is controlled by positive and negative cis-acting sequences.
Sequences from -3500 to +1365 of the chicken histone H5 gene have been analyzed for the presence of cis-acting elements in H5 expressing (transformed CFU-E) and non-expressing cells (fibroblasts). The region from -3500 to -115 had little effect on transcription. Proximal upstream sequences contain a negative element (UNE, -115 to -95), capable to also repress the activity of the heterologous HS...
متن کاملAnalysis of erythroid nuclear proteins binding to the promoter and enhancer elements of the chicken histone H5 gene.
The chicken erythroid proteins binding to the histone H5 5' promoter and 3' erythroid-specific enhancer regions were identified. In DNase I footprinting and gel mobility shift experiments with immature adult erythrocyte nuclear extracts, we have demonstrated the binding of proteins to the GC-box, a high affinity Sp1 binding site, and to the upstream promoter element. We have previously demonstr...
متن کاملThe effect of aspirin on the interaction of histone 05 and 05-DNA
The linker histones (H1 or H5) which play a key role in the folding of chromatin, are general repressors of gene expression. Nuclei of the mature chicken erythrocytes (and in some mammalian cells) contain both of them. Although the interaction of H5 with DNA is stronger than that of H1, it does not prevent the transcription of some erythroid-specific genes. It has been shown that some modificat...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)67271-0